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Gene cloning, expression and activity detection of porcine interleukin-18 mature protein in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology ; (12): 214-219, 2008.
Artículo en Chino | WPRIM | ID: wpr-276137
ABSTRACT
Porcine interleukin-18 mature protein gene was amplified from porcine spleen cells by RT-PCR. PCR product was cloned into the T vector pGEM-T for sequencing. The nucleotide sequence of this gene was 474 bp. Then, it was subcloned into the prokaryotic expressing plasmid vector pGEX6P-1 and transformed into host E. coli strain BL21 for expression. The expression of pIL-18 mature protein gene was identified by SDS-PAGE .The expression product was fusion protein with molecular weight of 45 kD and the percentage of expression protein in E. coil protein was 28%. The protein was purified by washing of inclusion bodies and the activity was measured by methyl thiazolyl tetrazolium (MTT).
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Porcinos / Proteínas Recombinantes de Fusión / Datos de Secuencia Molecular / Secuencia de Bases / Secuencia de Aminoácidos / Clonación Molecular / Interleucina-18 / Alergia e Inmunología / Escherichia coli / Genética Tipo de estudio: Estudio diagnóstico / Estudio pronóstico Límite: Animales Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2008 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Porcinos / Proteínas Recombinantes de Fusión / Datos de Secuencia Molecular / Secuencia de Bases / Secuencia de Aminoácidos / Clonación Molecular / Interleucina-18 / Alergia e Inmunología / Escherichia coli / Genética Tipo de estudio: Estudio diagnóstico / Estudio pronóstico Límite: Animales Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2008 Tipo del documento: Artículo