Gene cloning, expression and activity detection of porcine interleukin-18 mature protein in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 214-219, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-276137
ABSTRACT
Porcine interleukin-18 mature protein gene was amplified from porcine spleen cells by RT-PCR. PCR product was cloned into the T vector pGEM-T for sequencing. The nucleotide sequence of this gene was 474 bp. Then, it was subcloned into the prokaryotic expressing plasmid vector pGEX6P-1 and transformed into host E. coli strain BL21 for expression. The expression of pIL-18 mature protein gene was identified by SDS-PAGE .The expression product was fusion protein with molecular weight of 45 kD and the percentage of expression protein in E. coil protein was 28%. The protein was purified by washing of inclusion bodies and the activity was measured by methyl thiazolyl tetrazolium (MTT).
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Porcinos
/
Proteínas Recombinantes de Fusión
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Secuencia de Aminoácidos
/
Clonación Molecular
/
Interleucina-18
/
Alergia e Inmunología
/
Escherichia coli
/
Genética
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2008
Tipo del documento:
Artículo
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