The expression, purification and activity analysis of the rice chitinase gene in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
; (12): 188-192, 2008.
Article
en Zh
| WPRIM
| ID: wpr-276142
Biblioteca responsable:
WPRO
ABSTRACT
The cloned cDNA sequence of rice (Oryza sativa L. Cpslo17) chitinase gene Oschi was cloned, (which was registered in GenBank, the accession number: EU045451) ligated with the expression vector pGEX-4T-1, and transformed into E. coli BL21(DE3). The expression of Oschi was induced by IPTG, and the conditions were optimized. After purification the in vitro activity of Oschi chitinase was analyzed, and the results indicated that it could efficiently degrade chitin.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Oryza
/
Proteínas Recombinantes
/
Datos de Secuencia Molecular
/
Quitina
/
Quitinasas
/
Clonación Molecular
/
Escherichia coli
/
Genética
/
Metabolismo
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2008
Tipo del documento:
Article