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Study of the effect of hepatitis C virus core protein on interferon-induced antiviral genes expression and its mechanisms / 生物工程学报
Chinese Journal of Biotechnology ; (12): 1000-1004, 2007.
Artículo en Chino | WPRIM | ID: wpr-276172
ABSTRACT
To study the effect of HCV core protein on the interferon-induced antiviral genes expression and its mechanisms. Methods HepG2 cells were transiently transfected with HCV core protein expression plasmid and the blank plasmid respectively. RT-PCR was used to analyze the effect of HCV core protein on PKR and 2'-5'OAS expression. The effect of HCV core protein on ISRE-medicated gene expression was detected by luciferase activity assay. Western-blot assay was performed to observe the change of mRNA and protein levels of SOCS3, STAT1 and p-STAT1 following HCV core expression. In the presence of HCV core protein, the transcription of PKR and 2'-5' OAS are down-regulated. ISRE-medicated reporter gene expression and STAT1 phosphorylation were inhibited. The transcription and expression of SOCS3 were induced compared with blank plasmid-transfected group. In HepG2 cells, HCV core protein can down-regulate the expression of some interferon-induced antiviral genes, which involves the induction of SOCS3 and the inhibition of STAT1 phosphorylation.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Fisiología / Proteínas Quinasas / Transcripción Genética / 2',5'-Oligoadenilato Sintetasa / Células Tumorales Cultivadas / Transfección / Regulación hacia Abajo / Proteínas del Núcleo Viral / Interferón-alfa Límite: Humanos Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2007 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Fisiología / Proteínas Quinasas / Transcripción Genética / 2',5'-Oligoadenilato Sintetasa / Células Tumorales Cultivadas / Transfección / Regulación hacia Abajo / Proteínas del Núcleo Viral / Interferón-alfa Límite: Humanos Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2007 Tipo del documento: Artículo