Establishment of indirect ELISA diagnose based on the VP1 structural protein of foot-and-mouth disease virus (FMDV) in pigs / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 961-966, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-276179
ABSTRACT
The complete gene encoding the structural protein of FMDV(VP1) was subcloned into expression vector pPROex-HT, resulting in the fusion expression plasmid pPROexHT-VP1. After transformed into E. coli BL21(DE3) and induced by IPTG, the fusion protein was expressed in high level. Western blot was performed to confirm that the expressed fusion protein could specifically react with antiserum against FMDV. Based on the fusion protein further purified, a novel indirect ELISA (VP1-ELISA) was developed to detect FMDV antibody in pigs. Comparison between VPl-ELISA and the government standard kit (liquid phase block ELISA) showed the two methods had 96.25 percent agreement by detecting 80 serum samples, indicating that the indirect VP1-ELISA was specific and sensitive.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Porcinos
/
Sangre
/
Proteínas Recombinantes de Fusión
/
Ensayo de Inmunoadsorción Enzimática
/
Sensibilidad y Especificidad
/
Virus de la Fiebre Aftosa
/
Proteínas de la Cápside
/
Diagnóstico
/
Alergia e Inmunología
/
Escherichia coli
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2007
Tipo del documento:
Artículo
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