Study of target pegylated recombinant mutant human granulocyte colony stimulating factor / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 919-923, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-276186
ABSTRACT
Recombinant mutant human granulocyte colony stimulating factor (rmhG-CSF) was pegylated, purified and characterized. rhG-CSF was mutated in position 1,3,4,5,17, and cysteine was added in C-terminal. rmhG-CSF was pegylated by PEG-Mal 20000 and separated by ion-exchange chromatography, gel filtration chromatography. Analysis of SDS-PAGE showed thar the purity of the separated PEG-rmhG-CSF was greater than 95%. and in intro and in vivo bioactivity study showed that target modified PEG-rmhG-CSF kept full bioactivity which was better than traditional pegylation method, and longer half-life was proved in mice.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Polietilenglicoles
/
Proteínas Recombinantes
/
Señales de Clasificación de Proteína
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Química
/
Factor Estimulante de Colonias de Granulocitos
/
Cromatografía por Intercambio Iónico
/
Secuencia de Aminoácidos
/
Proteínas Mutantes
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2007
Tipo del documento:
Artículo
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