Analysis of primary metabolites of ranolazine in dog urine by LC-MS(n) / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1031-1038, 2012.
Artículo
en Chino
| WPRIM
| ID: wpr-276204
ABSTRACT
Ranolazine and metabolites in dog urine were identified by LC-MS(n). Dog urine samples were collected after ig 30 mg x kg(-1) ranolazine, then the samples were enriched and purified through solid-phase extraction cartridge. The purified samples were analyzed by LC-MS(n). The possible metabolites were discovered by comparing the full scan and SIM chromatograms of the test samples with the corresponding blanks. Seventeen phase I metabolites and fourteen phase II metabolites were identified in dog urine. Three metabolites were identified by comparing with the control article. The metabolites were formed via the following metabolic pathways O-demethylation, O-dearylation, hydroxylation, N-dealkylation, amide hydrolysis, glucuronidation and sulfation. The LC-MS(n) method is suitable for the rapid identification of drug and its metabolites in biologic samples.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Piperazinas
/
Orina
/
Administración Oral
/
Cromatografía Liquida
/
Espectrometría de Masa por Ionización de Electrospray
/
Espectrometría de Masas en Tándem
/
Extracción en Fase Sólida
/
Ranolazina
/
Acetanilidas
/
Metabolismo
Límite:
Animales
Idioma:
Chino
Revista:
Acta Pharmaceutica Sinica
Año:
2012
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS