Effect of heparin-derived oligosaccharide on vascular smooth muscle cell proliferation through inhibition of PKC-alpha expression / 药学学报
Acta Pharmaceutica Sinica
;
(12): 993-1000, 2012.
Artículo
en Inglés
| WPRIM
| ID: wpr-276210
ABSTRACT
In this study, the effect of heparin-derived oligosaccharide (HDO) on bovine vascular smooth muscle cell (VSMC) proliferation and signal transduction mechanism involved were investigated. The levels of PKC-alpha protein and mRNA were determined by cell-based ELISA, RT-PCR, Western blotting and immunocytochemical methods. Meanwhile, mRNA levels of c-jun, c-myc and c-fos were assayed by RT-PCR method. The results showed that HDO inhibited newborn calf serum (NCS)-induced expression of PKC-alpha and proto-oncogenes, which may be one of the mechanisms for the inhibition of VSMC proliferation by HDO. Flow cytometry analysis indicated that HDO blocked NCS-induced cell cycle progression by arresting cells at G0/G1 phase. The results imply that HDO inhibits VSMC proliferation by moderating the gene level of PKC-alpha, eventually inhibiting proto-oncogene mRNA expression and blocking G1/S transition.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oligosacáridos
/
Farmacología
/
ARN Mensajero
/
Heparina
/
Transducción de Señal
/
Ciclo Celular
/
Células Cultivadas
/
Fase G1
/
Proteínas Proto-Oncogénicas c-myc
/
Proteínas Proto-Oncogénicas c-jun
Límite:
Animales
Idioma:
Inglés
Revista:
Acta Pharmaceutica Sinica
Año:
2012
Tipo del documento:
Artículo
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