A method of HPRE synthesis via transcription by T7 RNA polymerase in vitro / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 808-810, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-276345
ABSTRACT
<p><b>OBJECTIVE</b>To synthesize highly pure HBV post-transcriptional regulatory element (HPRE) via transcription in vitro by T7 RNA polymerase.</p><p><b>METHODS</b>HPRE gene was amplified by PCR from a template containing HBV complete genomic DNA and cloned into plasmid pGEM-11zf. The cloned DNA sequence was transcribed by T7 RNA polymerase.</p><p><b>RESULTS</b>The construction of HPRE gene recombinant plasmid and production of HPRE via transcription in vitro was successful.</p><p><b>CONCLUSION</b>In vitro transcription by T7 RNA polymerase can be used to synthesize highly pure HPRE.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Transcripción Genética
/
Proteínas Virales
/
ARN Polimerasas Dirigidas por ADN
/
Procesamiento Postranscripcional del ARN
/
Empalme del ARN
/
Virus de la Hepatitis B
/
Proteínas de Unión al ARN
/
ADN Polimerasa Dirigida por ADN
/
Genética
Idioma:
Chino
Revista:
Chinese Journal of Hepatology
Año:
2005
Tipo del documento:
Artículo
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