Relationship between the different replication status of HBV and mutations in core promoter in mother and children infected by mother-to-infant transmission / 中华肝脏病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To understand the relationship between the different replication status of HBV and mutations in core promoter in mother and child infected by mother-to-infant transmission.</p><p><b>METHODS</b>Core promoter was amplified by PCR and cloned into pGEM-T vector with T-A cloning technique. The recombinant plasmid pGEM-PreS/S was confirmed by digestion with restriction enzyme Apa I and Sac I. Two clones were selected to sequence each patient.</p><p><b>RESULTS</b>Every pair of mother and child had same serotype and genotype and the homology of nucleotides encoding "a" determinant was 98 to 100%. The number of mutations in core promoter in patients with high replication status was more than that of low replication status. Mutations were distributed in BCP and Kunitz-type serine protease inhibitor region mainly. This difference was not associated with mother or child.</p><p><b>CONCLUSIONS</b>The different replication status of HBV is caused by mutations in core promoter in mother and child infected by mother-to-infant transmission and seems not to be associated with the development status.</p>