Determination of linarin in Yuye Jiedu granule by RP-HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To determine the content of linarin in Yuye Jiedu granule.</p><p><b>METHOD</b>A HPLC method was developed. The chromatographic conditions are as follows Luna C18 column and acetonitrile-0.05 mol x L(-1) phosphate buffer-phosphoric acid (30:70:0.06) as mobil phase, detection wavelenth at 327 nm.</p><p><b>RESULT</b>The linear range of linarin was 0.025-0.50 microg. The average recovery was 98.7% and RSD 2.7%.</p><p><b>CONCLUSION</b>The method is simple and accurate, with good repeatability, and can be used for determination of linarin in Yuye Jiedu granule.</p>