A new method for isolating CD34(+) cells based on complex of magnetic nanoparticles and antibody / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 537-541, 2007.
Article
en Zh
| WPRIM
| ID: wpr-276877
Biblioteca responsable:
WPRO
ABSTRACT
The purpose of this study was to synthesize the complex of magnetic nanoparticles and antibody, and to apply them to isolate the CD34(+) cells from umbilical cord blood, then to evaluate its separation efficiency. The complex of magnetic nanoparticles and antibody was used to separate CD34(+) cells from umbilical cord blood in the outer magnetic field because of its superparamagnetism, specific identification and function of combination with CD34(+) cells. Scanning electron microscopy was used to observe the morphology of the separated CD34(+) cells. Flow Cytometer was applied to evaluate the sorting efficiency of magnetic nanoparticles, liquid culture and colony culture were taken to assay proliferation and differentiation capacity of the separated CD34(+) cells. The results showed that the CD34(+) cells from umbilical cord blood were isolated fast and effectively by the complex of magnetic nanoparticles and monoclonal antibody. Moreover, the isolated CD34(+) cells still maintained its normal morphology, highly proliferative and differentiative capacity. It is concluded that the complex of magnetic nanoparticles and monoclonal antibody has been successfully synthesized and developed as a technique which efficiently and quickly isolates CD34(+) cells from umbilical cord blood.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Células Madre Hematopoyéticas
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Separación Inmunomagnética
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Antígenos CD34
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Biología Celular
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Alergia e Inmunología
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Nanopartículas
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Sangre Fetal
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Citometría de Flujo
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Magnetismo
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Metabolismo
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2007
Tipo del documento:
Article