Lidamycin induces apoptosis of human gastric carcinoma BGC823 cells and inhibits xenograft growth in nude mice / 药学学报
Acta Pharmaceutica Sinica
; (12): 601-604, 2008.
Article
en Zh
| WPRIM
| ID: wpr-277789
Biblioteca responsable:
WPRO
ABSTRACT
To investigate the effect of lidamycin (LDM) on human gastric carcinoma BGC823 cells and xenograft growth in nude mice, MTT (methyl thiazolyl tetrazolium) assay was used to determine the inhibition of BGC823 cell proliferation by LDM. Induction of apoptosis was studied by flow cytometry and TUNEL assay. The expression of VEGF was detected by Western blotting analysis. Athymic nude mice were used to determine in vivo antitumor activity. Proliferation inhibition and apoptosis induction were studied in lidamycin-treated cells. The expression of VEGF in BGC823 cells decreased in a dose-dependent manner. LDM at 0.02 mg x kg(-1) and 0.04 mg x kg(-1) suppressed the growth of BGC823 xenografts in nude mice by 57% and 72%, respectively. LDM potently induces apoptosis in human gastric carcinoma BGC823 cells and inhibits xenograft growth.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Patología
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Farmacología
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Neoplasias Gástricas
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Distribución Aleatoria
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Apoptosis
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Ensayos Antitumor por Modelo de Xenoinjerto
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Línea Celular Tumoral
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Factor A de Crecimiento Endotelial Vascular
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Proliferación Celular
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Relación Dosis-Respuesta a Droga
Tipo de estudio:
Clinical_trials
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Prognostic_studies
Límite:
Animals
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Female
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Humans
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Año:
2008
Tipo del documento:
Article