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Fluorescein Diacetate Microplate Assay in Cell Viability Detection / 中国医学科学院学报
Acta Academiae Medicinae Sinicae ; (6): 710-714, 2016.
Artículo en Inglés | WPRIM | ID: wpr-277916
ABSTRACT
Objective To investigate the application of the fluorescein diacetate (FDA) microplate assay in cell viability detection. Methods Cells were seeded in a 96-well culture plate until detection. After incubated with FDA,the plate was detected by fluorescence microplate analyzer. The effects of FDA incubation duration,concentration,and other factors on the assay's accuracy and stability were assessed. We also compared the results of FDA with methyl thiazolyl(MTT) in terms of cell numbers and HOinjury. Results Within 0-30 minutes,the fluorescence-cell number coefficient of FDA assay increased with duration and reached 0.99 in 27-30 minutes. The optimum concentration of final FDA in this study was 10-30 μg/ml. On cell viability detection,the result of FDA method was equivalent to MTT method. As to HOinjury assay,the sensitivity of FDA method was superior to MTT on the higher concentration HOtreatment due to a relative shorter duration for detection. Conclusion As a stable and reliable method,FDA is feasible for cell variability detection under varied conditions.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Coloración y Etiquetado / Bioensayo / Supervivencia Celular / Química / Fluoresceínas / Fluorescencia / Peróxido de Hidrógeno Tipo de estudio: Estudio diagnóstico Límite: Humanos Idioma: Inglés Revista: Acta Academiae Medicinae Sinicae Año: 2016 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Coloración y Etiquetado / Bioensayo / Supervivencia Celular / Química / Fluoresceínas / Fluorescencia / Peróxido de Hidrógeno Tipo de estudio: Estudio diagnóstico Límite: Humanos Idioma: Inglés Revista: Acta Academiae Medicinae Sinicae Año: 2016 Tipo del documento: Artículo