Experimental study on effect of sini decoction on myocardial endothelin in myocardial ischemic rats / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 610-612, 2002.
Artículo
en Chino
| WPRIM
| ID: wpr-278073
ABSTRACT
<p><b>OBJECTIVE</b>To study the molecular mechanism of effect of Sini Decoction (SND) on myocardial endothelin (MET) in myocardial ischemic rats.</p><p><b>METHODS</b>Sprague-Dawley rats were randomly divided into 3 groups, the normal control group, the ischemia group and the SND group. Myocardial ischemia was produced by pituitrin in the latter two groups. The content, immunohistochemical assay and gene expression of MET-1 were determined in all the three groups and compared.</p><p><b>RESULTS</b>The content of MET in the SND group was significantly lower than that in the ischemia group (P < 0.01). Immunohistochemical examination showed that MET-1 was mainly located at the cardiac muscle cells and vascular endothelial cells with the grey scale obviously lower in the ischemia group than that in the control group and the SND group (P < 0.01). While RT-PCR showed that the grey scale of PCR product band in the ischemia group was significantly higher than that in the other two groups (P < 0.01).</p><p><b>CONCLUSION</b>SND could significantly lower MET content, it may be related to the effect of SND in inhibiting MET-1 gene expression and protein synthesis.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Hormonas Neurohipofisarias
/
ARN Mensajero
/
Medicamentos Herbarios Chinos
/
Distribución Aleatoria
/
Ratas Sprague-Dawley
/
Isquemia Miocárdica
/
Endotelina-1
/
Genética
/
Fitoterapia
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Año:
2002
Tipo del documento:
Artículo
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