Molecular cloning of Plasmodium yoelii dynamin-like protein (PyDyn) gene and the immunological character of its domains / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 176-180, 2003.
Article
en Zh
| WPRIM
| ID: wpr-278102
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To identify and clone a new full ORF gene of PyDyn (Plasmodium yoelii dynamin-like protein), and examine the protection of their expression products.</p><p><b>METHOD</b>Using the P. yoelii Genome technology and RT-PCR.</p><p><b>RESULTS</b>The full ORF gene of PyDyn was amplified from mRNA of the erythrocytic stage of P. yoelii., three domains of PyDyn were expressed in E. coli., and the fairly positive immunogenicity of them was showed by IFA. The full ORF gene of PyDyn was 2,433 bp and encode 811 amino acids. Its Gene Bank access number is AF458071. PyDyn belongs to the dynamin-like protein family according to its property.</p><p><b>CONCLUSION</b>The new full ORF gene of PyDyn is obtained and identified; their expressed domains are probably new candidates for malaria vaccine.</p>
Texto completo:
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Índice:
WPRIM
Asunto principal:
Datos de Secuencia Molecular
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Plasmodium yoelii
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Vacunas Sintéticas
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Secuencia de Bases
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Proteínas Protozoarias
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Química
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Secuencia de Aminoácidos
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Clonación Molecular
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Genes Protozoarios
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Vacunas contra la Malaria
Límite:
Animals
Idioma:
Zh
Revista:
Acta Academiae Medicinae Sinicae
Año:
2003
Tipo del documento:
Article