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Bone marrow stem cells transplantation improve locomotive function of dystrophin/utrophin gene double knock-out mice / 中国医学科学院学报
Acta Academiae Medicinae Sinicae ; (6): 160-163, 2003.
Artículo en Chino | WPRIM | ID: wpr-278106
ABSTRACT
<p><b>OBJECTIVE</b>Study the improvement of locomotive faculty of dystrophin/utropin gene double knock-out mice (dko mice) by transplanting bone marrow stem cells.</p><p><b>METHODS</b>The bone marrow stem cells of C57BL/6 mice (4- to 5-weeks age) were cultured in vitro for three days, before transplanted intravenously (1.0 x 10(7) for each) into 11 dko mice (7- to 8-weeks age). The dko mice were irridiated with 7Gy gamma-ray before transplantation. 8-9 weeks after transplantation, the locomotroy function, electromyography items and expression of dystrophin in transplanted mice and controls were observed.</p><p><b>RESULTS</b>8-9 weeks after transplantation, the dropping times of hauling wire were 3.09 +/- 2.47, compared with that of the control dko mice(16.78 +/- 3.60), there are distinct differences. About electromyography items, the duration of active potential and amplitude of maxim contractions were (4.99 +/- 1.62) ms and(2872 +/- 1474.33) microV, compare with those of control dko mice(3.69 +/- 0.40) ms and(1210.0 +/- 551.0) microV, respectively, about 7% fibers of the muscle tissue of transplanted dko mice expressed dystrophin protein.</p><p><b>CONCLUSIONS</b>8-9 weeks after transplanted with homology bone marrow stem cells, the locomotive function and electromyography items of transplanted dko mice were obviously improved, and about 7% muscle tissue fibers of the mice expressing dystrophin protein were observed. It suggested that there is an ideal prospect for DMD therapy with bone marrow stem cells transplantation.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Cirugía General / Distrofina / Ratones Noqueados / Trasplante de Células Madre Hematopoyéticas / Distrofia Muscular de Duchenne / Proteínas del Citoesqueleto / Utrofina / Genética / Proteínas de la Membrana / Ratones Endogámicos C57BL Límite: Animales Idioma: Chino Revista: Acta Academiae Medicinae Sinicae Año: 2003 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Cirugía General / Distrofina / Ratones Noqueados / Trasplante de Células Madre Hematopoyéticas / Distrofia Muscular de Duchenne / Proteínas del Citoesqueleto / Utrofina / Genética / Proteínas de la Membrana / Ratones Endogámicos C57BL Límite: Animales Idioma: Chino Revista: Acta Academiae Medicinae Sinicae Año: 2003 Tipo del documento: Artículo