Construction of a collagen gel dermis with improved biological property / 中国医学科学院学报

ABSTRACT

<p><b>OBJECTIVE</b>To improve the biological property of artificial skin.</p><p><b>METHODS</b>We have ameliorated Hansburgh and Middelkoop's method of manufacturing artificial dermis. The type I collagenase and Dispase were used to isolated neonate prepuce' dermis fibroblast. The gel dermis was constructed by compounding the fibroblast and collagen swelling solution. The property of the collagen gel dermis was measured.</p><p><b>RESULTS</b>The neonate prepuce's dermis fibroblast had property of high proliferation, high activation of the dermis, and it could secrete abundant extracellular matrix (ECM).</p><p><b>CONCLUSION</b>The collagen gel dermis is an useful dermis substitute.</p>