Establishment and application of a RT-PCR system for quantifying mRNA of 4 hsp genes in human cells / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 321-324, 2002.
Artículo
en Chino
| WPRIM
| ID: wpr-278173
ABSTRACT
<p><b>OBJECTIVE</b>To establish a RT-PCR system for detecting mRNA expression level of 4 hsp genes in human cells.</p><p><b>METHODS</b>RT-PCR system was established with gene cloning, gene recombination, in vitro transcription and RT-PCR techniques; The detection of expression level of 4 hsp genes in SW13 cell was carried out with this system.</p><p><b>RESULTS</b>In SW13 cell, hsp70 and hsp90 alpha were typical heat shock induced genes, while hsp60 and hsp90 beta were efficiently expressed and further induced by heat-shock to various extent.</p><p><b>CONCLUSIONS</b>In our hands novel RT-PCR system can be used to detect mRNA expression level of 4 human hsp genes.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
ARN Mensajero
/
Células Tumorales Cultivadas
/
Leucemia de Células T
/
Proteínas HSP90 de Choque Térmico
/
Proteínas HSP70 de Choque Térmico
/
Chaperonina 60
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Proteínas del Choque Térmico HSP40
/
Genética
Límite:
Humanos
Idioma:
Chino
Revista:
Acta Academiae Medicinae Sinicae
Año:
2002
Tipo del documento:
Artículo
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