The role of p53 binding site on the trans binding of p53 to Hsp90 beta gene / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 285-288, 2002.
Artículo
en Chino
| WPRIM
| ID: wpr-278181
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of p53 binding site (+31/+60) of hsp90 beta gene on its transcriptional regulation.</p><p><b>METHODS</b>The binding site was first inserted into pBS-SK. After the plasmid annealing and elongation with mutagenic and selective primers, nuclease digestion and bacteria transformation was performed twice to select the positive mutated plasmid. Electrophoretic mobility shift assays (EMSA) was employed to detect the binding of hsp90 beta gene fragment containing mutated p53 binding site and Jurkat cell nuclear extract transfected by p53 expression vector.</p><p><b>RESULTS</b>The sequence analysis profile confirmed a successful mutation of two bases on the core sequence of the second half binding site. EMSA results showed the specific DNA-protein complex band disappeared after the mutation.</p><p><b>CONCLUSIONS</b>The core sequence of p53 binding site plays a key role in the trans binding of p53 to hsp90 beta gene.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Transcripción Genética
/
Sitios de Unión
/
Leucemia de Células T
/
Activación Transcripcional
/
Mutagénesis Sitio-Dirigida
/
Proteína p53 Supresora de Tumor
/
Proteínas HSP90 de Choque Térmico
/
Genética
/
Metabolismo
Límite:
Humanos
Idioma:
Chino
Revista:
Acta Academiae Medicinae Sinicae
Año:
2002
Tipo del documento:
Artículo
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