Effects of berberine on adiponectin mRNA expression in 3T3-L1 adipocyte / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore the effects of berberine and insulin on adiponectin mRNA expression in 3T3-L1 adipocyte.</p><p><b>METHOD</b>The 3T3-L1 adipocyte was treated with berberine and insulin for 48 hours, the level of adiponectin mRNA in 3T3-L1 adipocyte expression was determined with semiquantity RT-PCR using beta-actin as internal reference.</p><p><b>RESULT</b>The level of adiponetin mRNA in 3T3-L1 adipocyte was increased after treated with berberine only (P < 0.05), the effect of berberine was inhibited by high concentration insulin (P < 0.05).</p><p><b>CONCLUSION</b>In vitro, berberine increases the expression of adiponectin in 3T3-L1 adipocyte, insulin inhibits the effect of berberine.</p>