HPLC combined with PCA technology for analysis of five gingerol compounds in different processing degrees of ginger charcoal / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 4200-4204, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-279262
ABSTRACT
To establish a new method for simultaneously determining the content of five gingerol compounds in different processing degrees of ginger charcoal and PCA principal component analysis was conducted for analysis. Samples were analyzed on Ultimate TM XB-C18 column (4.6 mm x 250 mm, 5 μm) , with acetonitrile (A) -0.1% phosphoric acid solution (B) as mobile phase for gradient elution. Detection wavelength was set at 280 nm. The flow rate was 0.6 mL x min(-1) and the column temperature was 30 degrees C. The five compounds were separated well and showed good linearity (r ≥ 0.999 7) within the concentration ranges tested. The average value for recoveries was between 98.86% - 101.5% (RSD 1.4% - 2.9%). The contents of five compounds showed difference among different processing degrees of ginger charcoal. Zingiberone had the highest content in the standard carbon, and the content of gingerol was decreased as the deepening of processing degree. Different processing degrees of ginger charcoal were classified into three groups with PCA, and provided scientific basis for establishing the quality standards of ginger charcoal.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Medicamentos Herbarios Chinos
/
Catecoles
/
Carbón Orgánico
/
Química
/
Química Farmacéutica
/
Cromatografía Líquida de Alta Presión
/
Zingiber officinale
/
Análisis de Componente Principal
/
Alcoholes Grasos
/
Métodos
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2015
Tipo del documento:
Artículo
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