Studies on reversing MDR of K562/A02 by ramification of curcumin hydrolyzed / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2460-2463, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-279419
ABSTRACT
<p><b>OBJECTIVE</b>To determine the reversal effects of ramification of curcumin hydrolyzed on multidrug-resistant cell line K562/A02, and explore its reversal mechanism.</p><p><b>METHOD</b>After treatment with ramification of curcumin hydrolyzed, the sensitivity of K562/A02 cells to usuall chemotherapeutic drugs were determined by MTT. The expression of P-gp in K562/A02 and K562 cells was detected by immunohistochemical method. Intracellular mean fluorescence intensity (MFI) of DNR in K562 and K562/A02 cells was detected by Flow Cytometry.</p><p><b>RESULT</b>After treatment with the ramification of curcumin, hydrolyzed (2.5 mg x L(-1)) IC50, of usuall chemotherapeutic drugs to K562/A02 decreased. The sensitivity of K562/A02 cells increased. The expression of the P-gp in K562/A02 cells decreased (P < 0.05); MFI of the DNR in K562/A02 cells more significantly increased (P < 0.05). The expression of mdrl-mRNA decreased.</p><p><b>CONCLUSION</b>The ramification of curcumin hydrolyzed have effects on the reversal multidrug-resistant of K562/A02 cells in vitro. It could enhance the sensitivity of K562/A02 cells to chemotherapeutic drugs and the mechanism might be associated with inhibiting P-gp-mediated drug efflux and increasing of intracellular concentration of chemotherapeutic drugs.</p>
Texto completo:
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Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Medicamentos Herbarios Chinos
/
Expresión Génica
/
Química
/
Miembro 1 de la Subfamilia B de Casetes de Unión a ATP
/
Resistencia a Múltiples Medicamentos
/
Resistencia a Antineoplásicos
/
Células K562
/
Curcumina
/
Quimioterapia
Límite:
Humanos
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2010
Tipo del documento:
Artículo
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