Simultaneous determination of five active components in compound preparation of Dangguishuan by high-performance liquid chromatography / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2402-2405, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-279433
ABSTRACT
<p><b>OBJECTIVE</b>To develop a HPLC method for determination of matrine, oxymatrine; ferulic acid, L-shikonin and beta, beta-dimethylacrylshikonin in compound preparatioti of Dangguishuan.</p><p><b>METHOD</b>The chromatogtaphic separation was performed on a Kromasil ODS C18 column (4.6 mm x 250 mm, 5 microm) maintaining at 30 degrees C during the whole process. The mobile phase consisted of methanol and 0.1% triethylamine aqueous solution (adjusted with phosphoric acid, pH 3) at a flow rate of 1.0 mL x min(-1). The detection wavelength was set at 220 nm for matririne and oxymatrine, 316 nm for ferulic acid, 516 nm for L-shikonin and beta, beta-dimethylacrylshikonin, respectively.</p><p><b>RESULT</b>All the compounds showed good linearity (r > 0.9996) in the range of the test concentrations, and the average recoveries of the method is betwuen 96.92% and 102.22%, RSD < 3.1%.</p><p><b>CONCLUSION</b>The method is proved to be credible, sensitive, accurate and repeatable. It can be applied to determine of matrine, oxymatrine, ferulic acid, L-shikonin and beta, beta-dimethylacrylshikonin in compound preparation of Dangguishuan simultaneously, and provide a basal method of quality control to this preparation and other relative preparations.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Quinolizinas
/
Medicamentos Herbarios Chinos
/
Cromatografía Líquida de Alta Presión
/
Naftoquinonas
/
Ácidos Cumáricos
/
Alcaloides
/
Métodos
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2010
Tipo del documento:
Artículo
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