Comparison of two urinary protein preparation methods: nitrocellulose membrane preservation and acetone precipitation / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 982-989, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-279454
ABSTRACT
Nitrocellulose membrane based urinary protein preservation method is simple, fast and economic, but its advantage over the traditionally used acetone precipitation method is still unclear. In this work, we prepared urinary proteins by the two methods by LC-MS/MS. Then we used protein spectra counts to assess the reproducibility of the two methods. Proteins identified by the two methods were almost the same in number, spectral count distribution and distribution of coefficients of variation value. In conclusion, nitrocellulose membrane method is generally the same as acetone precipitation method. It can be used for large scale preservation of clinical urine samples.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Acetona
/
Espectrometría de Masas
/
Orina
/
Proteínas
/
Química
/
Reproducibilidad de los Resultados
/
Cromatografía Liquida
/
Colodión
/
Espectrometría de Masas en Tándem
Tipo de estudio:
Estudio pronóstico
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2014
Tipo del documento:
Artículo
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