Comparison of two urinary protein preparation methods: nitrocellulose membrane preservation and acetone precipitation / 生物工程学报
Chinese Journal of Biotechnology
; (12): 982-989, 2014.
Article
en Zh
| WPRIM
| ID: wpr-279454
Biblioteca responsable:
WPRO
ABSTRACT
Nitrocellulose membrane based urinary protein preservation method is simple, fast and economic, but its advantage over the traditionally used acetone precipitation method is still unclear. In this work, we prepared urinary proteins by the two methods by LC-MS/MS. Then we used protein spectra counts to assess the reproducibility of the two methods. Proteins identified by the two methods were almost the same in number, spectral count distribution and distribution of coefficients of variation value. In conclusion, nitrocellulose membrane method is generally the same as acetone precipitation method. It can be used for large scale preservation of clinical urine samples.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Acetona
/
Espectrometría de Masas
/
Orina
/
Proteínas
/
Química
/
Reproducibilidad de los Resultados
/
Cromatografía Liquida
/
Colodión
/
Espectrometría de Masas en Tándem
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2014
Tipo del documento:
Article