Crystallography of ATP hydrolysis mechanism in rat brain kinesin / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 644-657, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-279475
ABSTRACT
Rat brain kinesin is a conventional kinesin that uses the energy from ATP hydrolysis to walk along the microtubule progressively. Studying how the chemical energy in ATP is utilized for mechanical movement is important to understand this moving function. The monomeric motor domain, rK354, was crystallized. An ATP analog, AMPPNP, was soaked in the active site. Comparing the complex structure of rK354 x AMPPNP and that of rK354ADP, a hypothesis is proposed that Glu237 in the Switch II region sensors the presence of gamma-phosphate and transfers the signal to the microtubule binding region.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fosfatos
/
Unión Proteica
/
Encéfalo
/
Adenosina Trifosfato
/
Adenilil Imidodifosfato
/
Cinesinas
/
Dominio Catalítico
/
Cristalografía
/
Hidrólisis
/
Metabolismo
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2014
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS