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Retroviral vector-mediated HSVtk gene expression and acquisition of high titer recombinant virus / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology ; (6): 332-336, 2004.
Artículo en Chino | WPRIM | ID: wpr-279542
ABSTRACT
<p><b>OBJECTIVE</b>To explore the HSVtk gene expression mediated by the retroviral vector and to obtain high titer recombinant retroviral virus.</p><p><b>METHODS</b>The recombinant vector pRevTRE/HSVtk was constructed by inserting HSVtk gene into pRevTRE. The recombinant retrovirus, which was produced from cloned PA317 cells screened by hygromycin B after "micro-pingpong" technique transferring with pRevTRE/HSVtk plasmids DNA by using modified calcium phosphate precipitation method. HSVtk gene expression was performed on target cells and virus titers were detected in different cultured temper, time and sodium butyrate concentration.</p><p><b>RESULTS</b>The recombinant retroviral vector pRevTRE/HSVtk was constructed and HSVtk gene expression was detected on target cells after they were infected with the recombinant retrovirus.</p><p><b>CONCLUSION</b>High titer of retroviruses could be obtained in the culture medium of PA317 cell line through "micro-pingpong" technique at 30 hours and 10 mmol/L sodium butyrate concentration followed by frozen ultrafiltration.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Recombinación Genética / Retroviridae / Timidina Quinasa / Virología / Neoplasias de la Mama / Transfección / Regulación Enzimológica de la Expresión Génica / Línea Celular / Volumetría Límite: Animales / Femenino / Humanos Idioma: Chino Revista: Chinese Journal of Experimental and Clinical Virology Año: 2004 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Recombinación Genética / Retroviridae / Timidina Quinasa / Virología / Neoplasias de la Mama / Transfección / Regulación Enzimológica de la Expresión Génica / Línea Celular / Volumetría Límite: Animales / Femenino / Humanos Idioma: Chino Revista: Chinese Journal of Experimental and Clinical Virology Año: 2004 Tipo del documento: Artículo