Dynamic expression of matrix metalloproteinase-2, membrane type-matrix metalloproteinase-2 in experimental hepatic fibrosis and its reversal in rat / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 328-331, 2004.
Article
en Zh
| WPRIM
| ID: wpr-279543
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression dynamics and significance of matrix metalloproteinase-2 (MMP-2) membrane type-matrix metalloproteinase-2 (MT-MMP-2) in hepatic fibrosis and its reversal counterpart.</p><p><b>METHODS</b>An experimental CCl4 induced hepatic fibrosis rat model was established by intraperitoneal administration of carbon tetrachloride for 2, 4, 6, 8, 10 weeks, and normal rats were used as a control group. The immunohistochemical methods and in situ hybridization were used to detect MMP-2,MT-MMP-2 mRNA and related antigens in the liver.</p><p><b>RESULTS</b>MMP-2,MT-MMP-2 mRNA and related antigens were expressed in mesenchymal cells and parts of hepatocytes besides active pathological changes, especially in the fibrous septum and portal area. Expression of MMP-2,MT-MMP-2 mRNA and related antigens were increased in hepatic fibrosis and decreased gradually in its reversal counterpart.</p><p><b>CONCLUSION</b>This study suggested that mesenchymal cells are the main cellular origins of MMPs. The levels of MMP-2 and MT-MMP-2 antigens and gene expression were closely related to hepatic fibrosis. MMP-2 and MT-MMP-2 may play important roles in hepatic fibrosis and its reversal counterpart.</p>
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Asunto principal:
Patología
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ARN Mensajero
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Intoxicación por Tetracloruro de Carbono
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Regulación Enzimológica de la Expresión Génica
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Ratas Wistar
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Metaloproteinasa 2 de la Matriz
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Metaloproteinasas de la Matriz
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Hepatocitos
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Metaloproteinasas de la Matriz Asociadas a la Membrana
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Células Madre Mesenquimatosas
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2004
Tipo del documento:
Article