Preparation and characterization of monoclonal antibodies against SARS-associated coronavirus nucleocapsid protein / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 316-320, 2004.
Article
en Zh
| WPRIM
| ID: wpr-279546
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To obtain monoclonal antibodies (McAbs) against severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) nucleocapsid (N) protein to develop diagnostic test for SARS and study the pathogenesis of the disease.</p><p><b>METHODS</b>BALB/c mice were immunized with purified N protein of SARS-CoV. Hybridoma cell lines secreting monoclonal antibodies against SARS-associated coronavirus nucleocapsid were established after cell fusion with mouse splenic cells and SP2/0 cells. The specificity of the McAbs obtained was examined by Western blot and indirect fluorescence assay. Epitopes reacted with the McAbs were preliminarily located through Western blot by expressing truncated N proteins.</p><p><b>RESULTS</b>After cell fusion and three rounds of cell cloning, six hybridoma cell lines secreting monoclonal antibodies specifically against SARS-CoV nucleocapsid were obtained. Western blot and indirect fluorescence assay showed that the McAbs reacted specifically with nucleocapsid protein and SARS-CoV. Among the six McAbs, three recognize the epitopes located in the N-terminus of the protein, whereas the others reacted with those located in the C-terminus.</p><p><b>CONCLUSION</b>The anti-SARS-CoV nucleocapsid McAbs were developed and these McAbs may be useful in the development of diagnosis assays and basic research of SARS.</p>
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Índice:
WPRIM
Asunto principal:
Química
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Secreciones Corporales
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Proteínas de la Nucleocápside
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Coronavirus Relacionado al Síndrome Respiratorio Agudo Severo
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Alergia e Inmunología
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Hibridomas
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Ratones Endogámicos BALB C
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Anticuerpos Monoclonales
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Anticuerpos Antivirales
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Especificidad de Anticuerpos
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2004
Tipo del documento:
Article