Coxsackie virus B types were discriminated by RT-PCR / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop a method for detection of coxsackie B virus type 1-6 by RT-PCR.</p><p><b>METHODS</b>A pair of primers were designed to amplify all types of coxsackie B virus 1-6 efficiently. The PCR product was hybridized in micro-wells in which 6 type specific oligonucleotide probes had been coated respectively, colorimetric detection was performed to discriminate the types of coxsackie B virus.</p><p><b>RESULTS</b>This method was shown to be concordant with the IgM ELISA, 71.7% of anti-coxsackie B positive cases could be detected by RT-PCR.</p><p><b>CONCLUSION</b>The RT-PCR method can type coxsackie B virus efficiently and provides a tool for clinical diagnosis and epidemiological investigation.</p>