Application of fluorescent real-time reverse transcriptase-polymerase chain reaction in detecting influenza viruses / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To apply fluorescent real-time reverse transcriptase-polymerase chain reaction (RT-PCR) in detecting influenza viruses.</p><p><b>METHODS</b>A total of 207 oral swab samples were obtained in 16 collections from SARS patients and suspected influenza outbreak cases. They were subjected to influenza virus detection by fluorescent real-time RT-PCR, MDCK cell culture, and hemagglutinin inhibition assay.</p><p><b>RESULTS</b>Out of 207 samples, 79 (38.16%) were positive for influenza viruses when tested by fluorescent real-time PCR, and 62 (29.95%) positive when tested by MDCK cell culture. There was a statistically significant difference between them (chi square=8.64, P less than 0.005). From 104 cases in 9 collections dual serum samples were obtainable. When tested with hemagglutinin inhibition assay, 64 cases (61.54%) showed a 4-fold increase against H3N2 antigen.</p><p><b>CONCLUSION</b>This study showed that fluorescent real-time PCR is a reliable, sensitive, and fast method for detecting influenza viruses.</p>