Full-length sequence of hepatitis B virus isolated from high incidence hepatocellular carcinoma area-Longan county / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 272-276, 2004.
Artículo
en Chino
| WPRIM
| ID: wpr-279556
ABSTRACT
<p><b>OBJECTIVE</b>To understand full-length sequence of HBV isolated from high incidence hepatocellular carcinoma area-Longan county, Guangxi.</p><p><b>METHODS</b>The nested polymerase chain reaction (nPCR) was used for amplifying the whole HBV DNA in sera of asymptomatic carriers. The products were sequenced by clone sequencing and homological analysis.</p><p><b>RESULTS</b>This isolate contained 3 215 bases. The genotype was C and the serotype was adw. There were 40 point mutations in polymerase gene which made 11 amino acids change. There were 11,2 and 3 point mutations in PreS1, PreS2 and S gene respectively which made 3,1,1, amino acids change. Six point mutations including the double mutations (nt 1762 A to T, 1764 G to A) were found in X gene leading to 4 amino acids change. There were 13 point mutations in C gene which made 2 amino acids change. No mutation was found in a determinant and Pre C. The isolate was quite close to the isolate from Vietnamese in evolution while far from the genotype C isolates from Shanghai, Beijing and Tibet.</p><p><b>CONCLUSION</b>No special sequence was found in the isolate from high incidence hepatocellular carcinoma area, Longan county, Guangxi.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Virología
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Portador Sano
/
China
/
Virus de la Hepatitis B
/
Epidemiología
/
Incidencia
/
Sistemas de Lectura Abierta
/
Secuencia de Aminoácidos
Tipo de estudio:
Estudio de incidencia
/
Estudio pronóstico
Límite:
Adulto
/
Humanos
/
Masculino
País/Región como asunto:
Asia
Idioma:
Chino
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2004
Tipo del documento:
Artículo
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