An experimental study on circadian rhythm of the proliferative index of mandibular osteoblast in goats / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>The aim of this study was to investigate the circadian rhythm of the proliferative index of mandibular osteoblasts of goats in a normal situation.</p><p><b>METHODS</b>Twenty four healthy goats were selected and randomly divided into six groups. They were killed, and a piece of bone tissue with 2 cm x 2 cm x 0.5 cm was obtained from right mandibular mental foramen region at 000, 400, 800, 1200, 1600 and 2000 time points, after they had been fed for one week. The primary cell culture method was used to cultivate osteoblasts from the bone. The method of anti-5-bromodeoxyuridine monoclonal antibody and the flow cytometric analysis were used to detect the proliferative index of the mandibular osteoblasts at different time. The data were analyzed using the method of single cosine rhythmometry.</p><p><b>RESULTS</b>The proliferative indexes of the mandibular osteoblasts of goats were higher at the nighttime and lower in the daytime. The undulation of the proliferative indexes displayed a significant circadian rhythm (P < 0.01). The peak value was obtained at about 1944, and the lowest value at about 744.</p><p><b>CONCLUSION</b>The proliferative indexes of the mandibular osteoblasts of goats are not stable within 24 hours in a normal situation, but with significant circadian rhythm. There are different proliferative indexes at different time points.</p>