Effects of epidermal growth factors on the proliferation and metabolism of A/J mouse embryonic palatal cells / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>The purpose of this study was to investigate the effects of epidermal growth factors (EGFs) with different concentration on the OD, DNA, protein, and PGE2 of A/J mouse embryonic palatal shelves cells (A/J MEPC) isolated from embryonic palatal shelves.</p><p><b>METHODS</b>The mouse embryonic palatal shelves cells were grown in different 39 pores (or bottles) with 9 gradient concentrations of EGF (0.005, 0.010, 0.050, 0.100, 0.500, 1.000, 5.000, 10.000, 50.000 ng/ml), and four pores were prepared for the same concentration, then the OD, DNA, protein and PGE2 of A/J MEPC were measured after 1 day, 3 days and 5 days.</p><p><b>RESULTS</b>EGFs stimulated DNA and PGE2 synthesis of A/J MEPC, and augmented proliferation index (PIX). Their effects were very obvious in promoting the proliferation of A/J MEPC, when the concentration was 10.000 ng/ml.</p><p><b>CONCLUSION</b>EGF may be important in regulating proliferation and metabolism of embryonic palatal shelves cells.</p>