The Usefulness of Calretinin Immunohistochemistry for Rectal Suction Biopsy to Diagnose Hirschsprung’s Disease / 소아외과
Journal of the Korean Association of Pediatric Surgeons
; : 23-28, 2016.
Article
en Ko
| WPRIM
| ID: wpr-27976
Biblioteca responsable:
WPRO
ABSTRACT
PURPOSE: Hirschsprung’s disease (HD) is a congenital intestinal disorder with absence of ganglion cells in the intestinal muscle and submucosa. Diagnosis is based on histopathological study such as H&E, and acetylcholinesterase (AchE) immunohistochemistry. Calretinin immunohistochemistry was introduced as a new diagnostic method against limitations of other staining. The aim of this study is to investigate the usefulness of calretinin immunohistochemistry for the diagnosis of HD compared to H&E and AchE. METHODS: Ten patients with HD and 22 non-HD patients were included in the study. H&E staining, AchE and calretinin immunohistochemistry were performed in all 32 patients. All slides were evaluated by same single pathologist and the diagnostic value was calculated for each H&E stain, AchE immunohistochemical staining, and calretinin immunohistochemical staining. RESULTS: Calretinin method had sensitivity of 100% and specificity of 100% for diagnosis of HD. Its diagnostic accuracy was 100%. AchE staining showed 100% of specificity and 80% of sensitivity. Diagnostic accuracy of H&E staining was 56.3%. CONCLUSION: We concluded that calretinin immunohistochemistry is a very useful and valuable method to diagnosis HD patient.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Acetilcolinesterasa
/
Succión
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Biopsia
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Inmunohistoquímica
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Sensibilidad y Especificidad
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Ganglión
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Diagnóstico
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Calbindina 2
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Hematoxilina
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Enfermedad de Hirschsprung
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
Ko
Revista:
Journal of the Korean Association of Pediatric Surgeons
Año:
2016
Tipo del documento:
Article