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Association of SNP276 in adiponectin gene with type 2 diabetes mellitus and insulin sensitivity / 中华医学遗传学杂志
Chinese Journal of Medical Genetics ; (6): 698-701, 2005.
Artículo en Chino | WPRIM | ID: wpr-279964
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the distribution of SNP276 in adiponectin gene in Chinese Hans and its impact on type 2 diabetes mellitus and insulin sensitivity.</p><p><b>METHODS</b>The study population consisted of 417 Chinese Hans residents in Anhui province, including 141 subjects with normal glucose tolerance (NGT) and 276 with type 2 diabetes (T2DM). The islet beta-cell insulin secretion and tissue insulin sensitivity were assessed by formulae of homeostasis model assessment (HOMA-IR & HOMA beta). Firstly, polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was used to determine whether variation exists in APM1. Then, exact variation was detected by automated DNA direct sequencing.</p><p><b>RESULTS</b>The genotypes of APM1 SNP276 were 0.489 GG, 0.418 GT and 0.092 TT and the major allele was G (frequency=0.699) in subjects with NGT. The distributions of genotypes and alleles of SNP276 both displayed significant difference between NGT and T2DM groups (P=0.031 and 0.013). The SNP276 non-TT (TG+GG) genotype was associated with increased risk of T2DM (OR=2.447, 95%CI 1.067-5.612, P=0.035). In T2DM group, the subjects with SNP276 GG or GT genotype had higher body mass index, body fat content, fasting plasma glucose and HOMA-IR than did those with TT genotype (P < 0.05 or P < 0.01). Besides, GG genotype had higher systolic blood pressure (P=0.021). In NGT group, SNP276 non-TT carrier had increased body mass index, body fat content, waist hip ratio, fasting plasma insulin, oral glucose tolerance test 2 h plasma insulin and HOMA-IR when compared with TT genotype (P < 0.05 or 0.01).</p><p><b>CONCLUSION</b>SNP276 in APM1 was associated with T2DM and insulin sensitivity.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Sangre / Glucemia / Resistencia a la Insulina / Secuencia de Bases / Reacción en Cadena de la Polimerasa / Análisis de Secuencia de ADN / Polimorfismo Conformacional Retorcido-Simple / Predisposición Genética a la Enfermedad / Polimorfismo de Nucleótido Simple / Diabetes Mellitus Tipo 2 Límite: Adulto / Femenino / Humanos / Masculino Idioma: Chino Revista: Chinese Journal of Medical Genetics Año: 2005 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Sangre / Glucemia / Resistencia a la Insulina / Secuencia de Bases / Reacción en Cadena de la Polimerasa / Análisis de Secuencia de ADN / Polimorfismo Conformacional Retorcido-Simple / Predisposición Genética a la Enfermedad / Polimorfismo de Nucleótido Simple / Diabetes Mellitus Tipo 2 Límite: Adulto / Femenino / Humanos / Masculino Idioma: Chino Revista: Chinese Journal of Medical Genetics Año: 2005 Tipo del documento: Artículo