High resolution DNA typing for HLA-A, B, Cw genes by polymerase chain reaction-sequence specific oligonucleotide probes method in the Shenyang Han population / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 665-667, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-279974
ABSTRACT
<p><b>OBJECTIVE</b>To investigate, at the DNA level, the polymorphism of HLA-A, -B, -Cw genes in the Chinese of Han ethnicity in Shenyang.</p><p><b>METHODS</b>Hybridization with polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSOP) was used to determine HLA-A, -B and -Cw genotypes of 108 unrelated healthy individuals from a Chinese Han population. These Hans were born and living in the Shenyang area.</p><p><b>RESULTS</b>The numbers of alleles identified were 21 for HLA-A, 43 for HLA-B, and 23 for HLA-Cw. All the allele frequency distributions were consistent with the Hardy-Weinberg equilibrium.</p><p><b>CONCLUSION</b>Using molecular method, the present authors have analyzed the characteristic of HLA I distribution in a group of indigenous Hans in Shenyang and thus have provided more accurate gene data for use in related researches.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
ADN
/
Antígenos HLA-A
/
Antígenos HLA-B
/
Antígenos HLA-C
/
Sondas de Oligonucleótidos
/
Desequilibrio de Ligamiento
/
China
/
Reacción en Cadena de la Polimerasa
/
Alelos
/
Frecuencia de los Genes
Tipo de estudio:
Estudio pronóstico
Límite:
Adolescente
/
Adulto
/
Femenino
/
Humanos
/
Masculino
País/Región como asunto:
Asia
Idioma:
Chino
Revista:
Chinese Journal of Medical Genetics
Año:
2005
Tipo del documento:
Artículo
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