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The cloning and expression of apoptosis associated gene ANNEXIN A2 induced by p53 gene / 中华医学遗传学杂志
Chinese Journal of Medical Genetics ; (6): 661-664, 2005.
Artículo en Chino | WPRIM | ID: wpr-279975
ABSTRACT
<p><b>OBJECTIVE</b>To identify the relationship between p53-dependent apoptosis associated genes and tumor metastasis.</p><p><b>METHODS</b>mRNA differential display (mRNA DD) was adopted for gene cloning after the different metastatic potential lung cancer cell lines were infected by Adv-p53 (a reconstructed adenovirus encoding wild type p53 gene). RT-PCR, Northern blot and Western blot assays were used to confirm the result from mRNA DD.</p><p><b>RESULTS</b>After induction by p53 gene, the ANNEXIN A2 gene had differential expression in the cell lines; its level was down regulated in all the cells infected by Adv-p53 gene, especially in the Anip973 cell lines with high metastatic potential. RT-PCR, Northern blot and Western blot assays confirmed the consequence.</p><p><b>CONCLUSION</b>The experimental data suggest that the ANNEXIN A2 gene may relate to cellular apoptosis induced by p53 gene. The affirmative relationship between ANNEXIN A2 gene and p53 needs further investigation.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Datos de Secuencia Molecular / Secuencia de Bases / Transfección / Regulación Neoplásica de la Expresión Génica / Adenoviridae / Western Blotting / Northern Blotting / Proteína p53 Supresora de Tumor / Clonación Molecular / Apoptosis Límite: Humanos Idioma: Chino Revista: Chinese Journal of Medical Genetics Año: 2005 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Datos de Secuencia Molecular / Secuencia de Bases / Transfección / Regulación Neoplásica de la Expresión Génica / Adenoviridae / Western Blotting / Northern Blotting / Proteína p53 Supresora de Tumor / Clonación Molecular / Apoptosis Límite: Humanos Idioma: Chino Revista: Chinese Journal of Medical Genetics Año: 2005 Tipo del documento: Artículo