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Effects of Newcastle disease virus on the expression of survivin and cell cycle in human tongue squamous carcinoma TSCCa cells / 南方医科大学学报
Journal of Southern Medical University ; (12): 558-561, 2008.
Artículo en Chino | WPRIM | ID: wpr-280149
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of Newcastle disease virus (NDV) infection on the expression of survivin and cell cycle in human tongue squamous carcinoma TSCCa cells.</p><p><b>METHODS</b>The proliferation of TSCCa cells infected with NDV in vitro was evaluated by means of MTT assay, and survivin expression in the infected cells was detected using RT-PCR and Western blotting. Flow cytometry was performed to assess the changes in the cell apoptosis, cell cycle and cell proliferation index (PI) of the cells.</p><p><b>RESULTS</b>NDV infection resulted in decreased survivin expression and increased apoptosis of TSCCa cells, with reduced cell percentage in G2/M and S phases and lowered PI of the cells, showing significant differences from those of the negative control cells (P<0.05).</p><p><b>CONCLUSION</b>NDV infection can inhibit survivin expression, affect the cell cycle of TSCCa cells and induce their apoptosis.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Fisiología / Virología / Virus de la Enfermedad de Newcastle / Neoplasias de la Lengua / Carcinoma de Células Escamosas / Ciclo Celular / Western Blotting / Apoptosis / Reacción en Cadena de la Polimerasa de Transcriptasa Inversa Límite: Humanos Idioma: Chino Revista: Journal of Southern Medical University Año: 2008 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Fisiología / Virología / Virus de la Enfermedad de Newcastle / Neoplasias de la Lengua / Carcinoma de Células Escamosas / Ciclo Celular / Western Blotting / Apoptosis / Reacción en Cadena de la Polimerasa de Transcriptasa Inversa Límite: Humanos Idioma: Chino Revista: Journal of Southern Medical University Año: 2008 Tipo del documento: Artículo