Differentiation of bone marrow mesenchymal stem cells co-cultured with endothelial cells under shear stress / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 85-88, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-280258
ABSTRACT
Differentiation of bone marrow mesenchymal stem cells (BMSCs) co-cultured with endothelial cells (ECs) under shear stress was studied. BMSCs and ECs were co-cultured on the two sides of PET membrane, and 20 dyn/cm2 shear stress produced by parallel plate flow chamber was performed after 72 hours. Cell morphology was observed under phase-difference microscope, and the expressions of smooth muscle-alpha-actin (SM-alpha-actin), calponin and smooth muscle myosin heavy chain (SMMHC) of BMSCs were detected by fluorescence immunocytochemistry. The co-cultured BMSCs became smooth muscle-like cells gradually; after 24 hours, the BMSCs started to express SM-alpha-actin. After 48 hours, they expressed SM-alpha-actin and calponin obviously. After 72 hours, obvious expressions of SM-alpha-actin and calponin, but not of SMMHC, were detected. Further static co-culture had no effect on SM-alpha-actin, calponin and SMMH expression of BMSCs; after 24 hours, shear stress induced feeble expression of SM-alpha-actin and obvious expression of SMMHC in co-cultured BMSCs, but it had no effect on the expression of calponin. The results suggest that shear stress may potentiate the differentiation of BMSCs (co-cultured with ECs) into mature smooth muscle-like cells.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Estrés Mecánico
/
Proteínas de Unión al Calcio
/
Células de la Médula Ósea
/
Diferenciación Celular
/
Células Cultivadas
/
Actinas
/
Ratas Sprague-Dawley
/
Cadenas Pesadas de Miosina
/
Técnicas de Cocultivo
/
Biología Celular
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Biomedical Engineering
Año:
2009
Tipo del documento:
Artículo
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