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Research methods in protein-protein and protein-nucleic acid interactions and application in the study of human enterovirus A71 / 病毒学报
Chinese Journal of Virology ; (6): 587-593, 2014.
Artículo en Chino | WPRIM | ID: wpr-280323
ABSTRACT
Methods for analyses of protein-protein interactions include yeast two hybrid (Y2H), phage dis- play (PD), co-immunoprecipitation (Co-IP), glutathione S-transferase pull-down (GST pull-down), cellular co-localization, far-western blotting, virus overlay protein binding assay (VOPBA), surface plasmon resonance (SPR), and fluorescence resonance energy transfer (FRET). Technologies for the detection of protein-nucleic acid interactions include yeast one hybrid (Y1H), chromatin immunoprecipitation (ChIP), electrophoretic mobility shift assay (EMSA), Southwestern blotting, reporter gene, Co-IP, GST pull-down, and PD. These methods are often used in the study of the human enterovirus A71 (EV-A71) by our research team. Reviews in the Chinese literature in this field are lacking, so we reviewed applications of these methods in the study of EV-A71. This review may impart important knowledge in the research of other viruses with regard to protein-protein and protein-nucleic acid interactions.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Proteínas Virales / ARN Viral / Química / Técnicas del Sistema de Dos Híbridos / Enterovirus Humano A / Ensayo de Cambio de Movilidad Electroforética / Transferencia Resonante de Energía de Fluorescencia / Genética / Metabolismo Idioma: Chino Revista: Chinese Journal of Virology Año: 2014 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Proteínas Virales / ARN Viral / Química / Técnicas del Sistema de Dos Híbridos / Enterovirus Humano A / Ensayo de Cambio de Movilidad Electroforética / Transferencia Resonante de Energía de Fluorescencia / Genética / Metabolismo Idioma: Chino Revista: Chinese Journal of Virology Año: 2014 Tipo del documento: Artículo