Calcineurin subunit B is not an effective adjuvant when combined with a novel HBV protein particle vaccine / 病毒学报
Chinese Journal of Virology
; (6): 554-560, 2014.
Article
en Zh
| WPRIM
| ID: wpr-280328
Biblioteca responsable:
WPRO
ABSTRACT
To compare different adjuvant formulation and explore the impact of Calcineurin B subunit(CnB) as adjuvant with a novel HBV protein particle (HBSS1) vaccine in mice, female C57BL/6 mice were immunized HBSS1 with Al(OH)3 only, or a normal dose (5 μg) CnB only, or (CnB+ Al(OH)3) mixture as the adjuvant. All immunized groups were primed twice at 4-week intervals; followed by boosting with recombinant adenoviral based HBV vaccine(rAdSS1) at 10-week intervals. We detected the antigen specific humoral response in mice, including total IgG antibody and IgG subtyping. Then, we characterized the specific cell-mediated immune (CMI) response by detection of γ-interferon secreting splenocytes after stimulaton with S or PreS1 peptide pools. No enhancement of immunity was found among the mice with 5 μg of CnB alone or combined with Al(OH), adjuvanted vaccine,which could not induce higher level of anti-PreS1 and anti-S antibodies and CMI than that of HBSS1 alone or Al(OH)3 adjuvanted vaccines. We concluded that CnB is not an effective adjuvant for a novel HBV subunit vaccine.
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Índice:
WPRIM
Asunto principal:
Farmacología
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Adyuvantes Inmunológicos
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Vacunas contra Hepatitis B
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Calcineurina
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Subunidades de Proteína
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Alergia e Inmunología
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Ratones Endogámicos C57BL
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Virology
Año:
2014
Tipo del documento:
Article