Construction of pEGFP-C1/U6-mediated plasmid expressing MDR1 shRNA / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 384-387, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-280660
ABSTRACT
To construct a plasmid expressing MDR1 short hairpin RNA (shRNA) mediated by pEGFP-C1/U6 vector, two coding sequences of 19 nucleotides were selected from MDR. Two pairs of oligonucleotides were designed for these two fragments. After annealing the formed double-stranded DNAs were ligated with plasmid pEGFP-C1/U6 (pEGFP-C1 vector with U6 promoter). The plasmids producing MDR1 shRNA were constructed from the inverted motif containing 9 spacers and four Ts. The results showed that the constructed plasmids were named pEGFP-C1/U6/A and pEGFP-C1/U6/B, and the constructs were identified by restriction and sequence analysis, no any base mutation was observed. It is concluded that plasmids of pEGFP-C1/U6/A and pEGFP-C1/U6/B expressing MDR1 shRNA were successfully constructed, providing a highly effective method for reversing the multidrug resistance in clinic.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plásmidos
/
ARN Mensajero
/
ARN Nuclear Pequeño
/
Miembro 1 de la Subfamilia B de Casetes de Unión a ATP
/
Resistencia a Múltiples Medicamentos
/
Resistencia a Antineoplásicos
/
ARN Interferente Pequeño
/
Proteínas Fluorescentes Verdes
/
Proteínas de Unión al ADN
/
Vectores Genéticos
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2006
Tipo del documento:
Artículo
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