Influence of human mesenchymal stem cells on cell proliferation and chemo-sensitivity of K562 cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 308-312, 2006.
Article
en Zh
| WPRIM
| ID: wpr-280677
Biblioteca responsable:
WPRO
ABSTRACT
This study was aimed to compare K562 cell proliferation, chemo-sensitivity and alteration of MDR1 before and after adhesive culture with MSC, so as to evaluate the relationship between chemodrug-resistance of leukemia cells and hemopoietic microenvironment. K562 cell cultivated in suspension and adhesively cultivated with MSC were collected respectively and cell proliferation curves were drawn; the cell cycle was determined by flow cytometry; the effect of chemotherapy on cellular viability and apoptosis of K562 cell was investigated, the MDR1 gene expression was determined by RT-PCR. The results showed that K562 cells adhesively cultivated with MSC were inhibited and cells in G0/G1 increased (P < 0.05), cells in S phase decreased (P < 0.05) and those in G0/G1 increased (P < 0.01), compared with that cultivated in suspension. In process of daunomycin-inducing apoptosis, K562 cell apoptosis in the adhesive culture with MSC was inhibited (P < 0.05). MDR1 gene expression in K562 cells was not induced or altered by adhesive co-cultivation. It is concluded that by co-culture of cell-cell contact with MSC, growth suppression and induction of chemo-resistance of K562 cells take place. The mechanism, however, seems not relevant with MDR1.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Farmacología
/
Fisiología
/
Células de la Médula Ósea
/
Daunorrubicina
/
Células Cultivadas
/
Apoptosis
/
Miembro 1 de la Subfamilia B de Casetes de Unión a ATP
/
Resistencia a Múltiples Medicamentos
/
Resistencia a Antineoplásicos
/
Técnicas de Cocultivo
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2006
Tipo del documento:
Article