Correlation analysis and identification of G421C in regulatory region of CYP4F2 gene with essential hypertension / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 143-147, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-281244
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the correlation between G421C polymorphism in the regulatory region of CYP4F2 gene and essential hypertension and its molecular mechanism.</p><p><b>METHODS</b>Totally 196 hypertensive patients (hypertension group) and 219 normotensive subjects (control group) were genotyped by polymerase chain reaction-restriction fragment length polymorphism. The promoter activity with different alleles was evaluated by reporter assay. A Myb responsive element was identified using gel retardation assay.</p><p><b>RESULTS</b>Significant differences were found in distribution of genotype and allele frequency of G421C between hypertension group and control group (P < 0.05), and homozygous GG genotype was independently associated with hypertension after adjustment for age, gender, body mass index, and other risk factors (odds ratios 1.87, 95% CI 1.11-3.13, P < 0.05). 421G reporter construct showed decreased promoter activity compared with 421C reporter construct. 421G existed in Myb responsive element, whereas 421C damaged this motif.</p><p><b>CONCLUSION</b>G421C polymorphism in the regulatory region of CYP4F2 gene is correlated with essential hypertension. 421G allele inhibits transcription by binding affinity of Myb responsive element.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Polimorfismo Genético
/
Estudios de Casos y Controles
/
Secuencias Reguladoras de Ácidos Nucleicos
/
Predisposición Genética a la Enfermedad
/
Elementos de Respuesta
/
Genes myb
/
Sistema Enzimático del Citocromo P-450
/
Familia 4 del Citocromo P450
/
Frecuencia de los Genes
/
Genética
Tipo de estudio:
Estudio observacional
/
Estudio pronóstico
/
Factores de riesgo
Límite:
Adulto
/
Femenino
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Acta Academiae Medicinae Sinicae
Año:
2006
Tipo del documento:
Artículo
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