X-ray irradiation enhances tumor necrosis factor receptor p55 expression in human colorectal cancer cells and inhibits the release of its soluble form in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1921-1923, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-281503
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of X ray on human colorectal cancer cells for their tumor necrosis factor receptor-p55 (TNFR-p55) expression and release of soluble soluble TNFR-p55 (sTNFR-p55) in vitro.</p><p><b>METHODS</b>The protein expression of TNFR-p55 in Lovo cells exposed to X-ray was detected using immunohistochemistry, and enzyme-linked immunosorbent assay was used to examine the levels of sTNFR-p55 in the supernatants of the cell culture. The cell apoptosis of the exposed cells was analyzed with flow cytometry, and the changes in cell morphology were observed microscopically.</p><p><b>RESULTS</b>X-ray exposure of cells resulted in a strong increase in TNFR-p55 expression of (P<0.01) and LoVo cell apoptosis (P<0.05). The levels of sTNFR-p55 in the supernatant of cells with X-ray exposure was significantly lowered in comparison with the levels before exposure (P<0.01). Optical microscopy showed that the exposed LoVo cells shrank and became spherical with cytoplasmic condensation and nuclear pyknosis.</p><p><b>CONCLUSION</b>X-ray exposure can induce LoVo cell apoptosis by increasing TNFR-p55 expression on the cell membrane and inhibiting the release of sTNFR-p55 in the supernatants.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Rayos X
/
Ensayo de Inmunoadsorción Enzimática
/
Inmunohistoquímica
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Neoplasias Colorrectales
/
Regulación Neoplásica de la Expresión Génica
/
Apoptosis
/
Línea Celular Tumoral
/
Receptores Tipo I de Factores de Necrosis Tumoral
/
Receptores Señuelo del Factor de Necrosis Tumoral
/
Citometría de Flujo
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2007
Tipo del documento:
Artículo
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