Effect of bleomycin A5 on the hemangioma-derived endothelial cell line XTPS-1 / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 324-328, 2012.
Artículo
en Chino
| WPRIM
| ID: wpr-281589
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of bleomycin A5 on the hemangioma-derived endothelial cell line XTPS-1.</p><p><b>METHODS</b>Hemangioma-derived endothelial cell line XTPS-1 was cultured with different concentration of bleomycin A5 (1000, 100, 10, 1, 0 mg/L), and then the survival rate was measured by methyl thiazolyl terazolium (MTT), the variation of cell morphology was observed using inverted phase contrast microscope and electron microscope, the variation of cell cycle and apoptosis rate were measured using flow cytometry.</p><p><b>RESULTS</b>After 24 hours culture the cell survival rate was (92.96 ± 3.66)% and (99.86 ± 0.12)% in lower saturation group (10 and 1 mg/L), but (34.08 ± 3.11)% and (43.28 ± 2.88)% in higher saturation group (1000 and 100 mg/L). The difference between them was more significant (P < 0.01). Lower saturation of bleomycin A5 (10 and 1 mg/L)could induce apoptosis but had almost no cytotoxic effect. Higher saturation of bleomycin A5 (1000 and 100 mg/L) not only induced apoptosis, but also had strong cytotoxic effect, which was concentration dependent.</p><p><b>CONCLUSIONS</b>bleomycin A5 could induce apoptosis, inhibit cell proliferation and has direct cytotoxic effect.</p>
Texto completo:
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Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Bleomicina
/
Ciclo Celular
/
Supervivencia Celular
/
Microscopía de Contraste de Fase
/
Apoptosis
/
Línea Celular Tumoral
/
Células Endoteliales
/
Microscopía Electrónica de Transmisión
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Stomatology
Año:
2012
Tipo del documento:
Artículo
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