Expression patterns of amelogenin and enamelin in developing mouse tooth germs / 中华口腔医学杂志
Chinese Journal of Stomatology
; (12): 173-176, 2012.
Article
en Zh
| WPRIM
| ID: wpr-281637
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To invesitgate the expression patterns of amelogenin and enamelin in the developing tooth germs.</p><p><b>METHODS</b>Mandible sections of postnatal day 1, 3, 7 and 14 mouse were prepared, immunohistochemical analysis and reverse transcriptase polymerase chain reaction (RT-PCR) were performed to detect the expression patterns of amelogenin and enamelin in mandibular first molars.</p><p><b>RESULTS</b>Amelogenin was observed in the cytoplasm of secretory ameloblasts and the whole enamel matrix layer. It was also transiently expressed in the odontoblasts of postnatal day 1 molars. Enamelin proteins were observed in the enamel layer deposited by secretory ameloblasts, especially intense beneath the ameloblast process and dentino-enamel junction. The mRNA levels of both amelogenin and enamelin were highest on postnatal day 7 (the ratio to glyceraldehyde phosphate dehydrogenase of amelogenin and enamelin: 0.813 ± 0.085 and 0.799 ± 0.064, respectively, P < 0.05).</p><p><b>CONCLUSIONS</b>Amelogenin and enamelin were enamel matrix proteins predominately expressed by secretory ameloblasts. The temporal-spatial expression patterns of amelogenin and enamelin indicate the important roles they played in amelogenesis and biomineralization.</p>
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Índice:
WPRIM
Asunto principal:
Factores de Tiempo
/
Germen Dentario
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ARN Mensajero
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Esmalte Dental
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Proteínas del Esmalte Dental
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Amelogenina
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Ameloblastos
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Amelogénesis
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Genética
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Metabolismo
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Stomatology
Año:
2012
Tipo del documento:
Article