Study on preparation of icaritin by enzymolysis of icariin with snail hydrolase / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To optimize the conditions for enzymolysis of Icariin by snail hydrolase.</p><p><b>METHOD</b>Take conversion rate as index, the effects of pH, temperature, reaction time, dosage of enzyme, concentration of Icariin and metal ion on hydrolysis were studied by single-factor designs and a L9 (3(4)) orthogonal design. The product was characterized through MS, 1H-NMR and 13C-NMR methods.</p><p><b>RESULT</b>The optimum enzymolysis conversion rate was achieved at 37 degrees C, pH 6.0 acetic acid-sodium acetate buffer solution and 48 h. The Ca2+, Mg2+ and Zn2+ had activation on helicase slightly but the Fe2+ inhibited enzymolysis significantly. The production was presumed as icaritin on the basis of spectral evidences.</p><p><b>CONCLUSION</b>Snail hydrolase can be used for preparing of icaritin. The condition is mildness and suitable for industrialization.</p>