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Genetic characterization of the non-structural protein NSP4 from epidemic strains of human rotavirus in China / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology ; (6): 10-14, 2003.
Artículo en Chino | WPRIM | ID: wpr-281866
ABSTRACT
<p><b>BACKGROUND</b>To clarify the features of gene variation among epidemic strains of human rotavirus NSP4 in China.</p><p><b>METHODS</b>SP4 genes from 27 epidemic strains of human rotavirus isolated in different area of China in recent years were amplified with RT-PCR, the resulted cDNAs were cloned and sequenced. The sequences of full length cDNAs were compared with 10 rotavirus NSP4 sequences available in the GenBank using the Clustal x 1.8 TreeView32 and DNA Star softwares. The G serotype of VP7 was analysed by PCR.</p><p><b>RESULTS</b>The homology of the amino acid among the 27 rotavirus strains isolated in China was 81.7%-99.4%. Based on the variation of amino acid sequence, the virus strains can be divided into two groups, represented by Wa and KUN with the homology of 92.0%-99.4% and 92.0%-98.9% within each group, respectively. The diversity between the two groups were 16.6%-21.0%. The Wa group could further be separated into three subgroups, according to the diversity between those strains and the characterization in the highly variable domain. The association between VP7 serotype and NSP4 genotype was not strong.</p><p><b>CONCLUSIONS</b>The NSP4 gene of human rotavirus epidemic strains in China can be divided into Wa and KUN two groups, Wa group is the main group and contains three subgroups possessing characteristic amino acid sites. Samples isolated in the same year but not in the same area shared higher homology.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Filogenia / Toxinas Biológicas / Variación Genética / Virología / ADN Viral / Glicoproteínas / Proteínas no Estructurales Virales / Análisis de Secuencia de ADN / ADN Complementario / Rotavirus Límite: Humanos Idioma: Chino Revista: Chinese Journal of Experimental and Clinical Virology Año: 2003 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Filogenia / Toxinas Biológicas / Variación Genética / Virología / ADN Viral / Glicoproteínas / Proteínas no Estructurales Virales / Análisis de Secuencia de ADN / ADN Complementario / Rotavirus Límite: Humanos Idioma: Chino Revista: Chinese Journal of Experimental and Clinical Virology Año: 2003 Tipo del documento: Artículo