Correlation between ITS genotype and geographical distribution of Pogostemon cablin / 药学学报

ABSTRACT

To investigate the correlation between genotype and distribution of Pogostemon cablin by sequencing ITS1 and ITS2 genes, and provide molecular information for its germplasm evaluation, ITS1 and ITS2 genes of Pogostemon cablin from different localities were identified by PCR direct sequencing. The sequences of ITS1 and ITS2 genes were 424 bp and 380 bp in length, respectively. And nineteen base substitutions were observed in ITS1 gene, and five in ITS2 gene. The results showed a good correlation between genotype and distribution of Pogostemon cablin, and ITS gene sequencing could provide useful molecular information for germplasm evaluation of the plant species verification.